微量元素与健康研究

目的 将矿物药磁石制备成纳米颗粒，进而研制出一款性能优异、具有止痛止血与抗炎作用的纳米药磁静电纺丝膜。方法 以静电纺丝的电压、质量比、乙酸浓度、纺丝液体积为自变量，以纳米纤维膜的表面形貌、水中形态、显微形态为评价指标，优选纳米纤维膜的最佳纺丝条件。基于纳米纤维膜的最佳纺丝条件，将纳米纤维膜纺丝液中加入不同质量分数纳米磁石混悬剂进行混纺，通过表面形貌、水中形态、机械性能检测以及吸水保湿实验对不同质量分数的纳米药磁纤维膜进行综合评价。结果 采用单因素实验考察得到纳米纤维膜最佳纺丝条件为：电压26 kV、(CS/PVA)质量比1∶9、乙酸浓度50%、体积15 mL。含有2%磁石的纳米药磁膜表面平整光滑，分布均匀且具有韧性；在水中浸泡后，膜上的水珠小而疏，平整展开无卷曲。与其他不同质量分数纳米药磁纤维膜相比，2%纳米磁石的纤维膜机械性能、吸湿性能与保湿性能均更优。结论 含2%磁石的纳米药磁纤维膜性能最优，单因素实验和静电纺丝法优选纤维膜的制备工艺简便快捷、准确可靠。纳米药磁纤维膜可用于伤口愈合的敷料，起到止痛、止血与抗炎作用，具有实际应用价值，也为其他止痛、止血与抗炎药物的实际应用提供参考，为矿物药的进一步开发与应用提供新的路径。

Objective To prepare the mineral medicine magnetite as nanoparticles and subsequently develop a magnetite-loaded electrospun nanofibrous membrane with favorable properties and analgesic, hemostatic, and anti-inflammatory effects.Methods Electrospinning voltage, CS/PVA mass ratio, acetic acid concentration, and spinning-solution volume were selected as independent variables, while surface morphology, morphology in water, and microscopic morphology of the nanofibrous membranes were used as evaluation parameters to optimize the electrospinning conditions. Under the optimized electrospinning conditions, nanosized magnetite suspensions at different mass fractions were incorporated into the spinning solution to prepare magnetite-loaded nanofibrous membranes. The membranes containing different mass fractions of nanosized magnetite were comprehensively evaluated in terms of surface morphology, morphology in water, mechanical properties, water absorption, and moisture-retention properties.Results Single-factor experiments identified the optimal electrospinning conditions as follows: voltage, 26 kV; CS/PVA mass ratio, 1:9; acetic acid concentration, 50%; and spinning-solution volume, 15 mL. The nanofibrous membrane containing 2% magnetite exhibited a smooth, flat, and uniformly distributed surface structure with good flexibility. After immersion in water, the water droplets on the membrane surface were small and sparsely distributed, and the membrane remained flat without curling. Compared with magnetite-loaded nanofibrous membranes containing other mass fractions of nanosized magnetite, the membrane containing 2% nanosized magnetite exhibited superior mechanical properties, water absorption, and moisture-retention properties.Conclusion The nanofibrous membrane containing 2% magnetite exhibited the best overall performance. Optimization of the preparation process using single-factor experiments and electrospinning was simple, rapid, accurate, and reliable. The magnetite-loaded nanofibrous membrane may be used as a wound-healing dressing with analgesic, hemostatic, and anti-inflammatory effects and therefore has potential practical applications. This study also provides a reference for the practical application of other analgesic, hemostatic, and anti-inflammatory agents and offers a new approach for the further development and utilization of mineral medicines.