微量元素与健康研究

目的 考察不同的乙醇浓度、浸渍时间和渗漉液收集量对生发酊的制备的影响，优选最佳的制备工艺。本实验通过观察复方生发酊对硫化钠致小鼠脱毛后的影响，对其治疗斑秃的药效进行初步评价。方法 用L₉(3⁴)正交试验和综合评分法考察渗漉乙醇的浓度、浸渍时间、渗漉液的收集量，生发酊最佳制备工艺。用硫化钠乙醇溶液建立小鼠斑秃模型，观察其毛发生长状况，并通过HE染色分析小鼠病理皮肤组织毛囊生长情况。结果 渗漉乙醇浓度65%、浸渍时间24 h、收集渗漉液100 mL为最佳制备工艺。生发酊中剂量组(生药浓度100 mg/mL)和米诺地尔擦剂组小鼠背部脱毛区域新生毛发的长度、新生毛发的重量均有明显增加，脱毛区域的皮肤组织毛囊增生比较活跃，毛囊数增多；空白组、模型组、低剂量组以及高剂量组小鼠背部脱毛区域新生毛发长度、重量均无明显变化。结论 本生发酊的制备工艺合理可行，生发酊中复方生发酊中剂量组剂量(生药浓度100mg/mL)对治疗脱发有一定的促毛发生长作用。

Objective To investigate the effects of ethanol concentration, maceration time, and percolate collection volume on the preparation of Compound Shengfa Tincture and to optimize its preparation process. In addition, the effects of Compound Shengfa Tincture on hair regrowth in a sodium sulfide-induced mouse model of alopecia were evaluated to preliminarily assess its therapeutic efficacy against alopecia areata.Methods An L₉(3⁴) orthogonal experimental design combined with comprehensive scoring was employed to investigate the effects of ethanol concentration for percolation, maceration time, and percolate collection volume and to determine the optimal preparation process for Compound Shengfa Tincture. A mouse model of alopecia was established using an ethanolic sodium sulfide solution. Hair regrowth was observed, and hair follicle growth in skin tissues from the depilated area was evaluated histopathologically by hematoxylin and eosin(H&E) staining.Results The optimal preparation conditions were a percolation ethanol concentration of 65%, a maceration time of 24 h, and a percolate collection volume of 100 mL. In the medium-dose Compound Shengfa Tincture group(crude drug concentration, 100 mg/mL) and the topical minoxidil group, both the length and weight of newly grown hair in the depilated dorsal area were markedly increased. Histopathological examination revealed active hair follicle proliferation and an increased number of hair follicles in the depilated skin. In contrast, no marked changes in the length or weight of newly grown hair were observed in the control, model, low-dose, or high-dose groups.Conclusion The optimized preparation process for Compound Shengfa Tincture is feasible and appropriate. At a crude drug concentration of 100 mg/mL, Compound Shengfa Tincture exhibited a hair growth-promoting effect in the mouse model, suggesting its potential for the treatment of hair loss.