18 | 0 | 63 |
下载次数 | 被引频次 | 阅读次数 |
目的 探讨NaAsO2对人正常肝L-02细胞凋亡的影响及BNIP3在NaAsO2致L-02细胞凋亡中的作用。方法 L-02细胞染不同浓度NaAsO2(0、5、10、20、40μmol/L)24 h。提取细胞总蛋白;采用流式细胞术检测各组L-02细胞凋亡水平;采用Western blot测定各组L-02细胞Caspase-3、BNIP3蛋白的表达;提取细胞RNA,qPCR检测BNIP3 mRNA的表达。结果 随着砷浓度增加,L-02细胞凋亡水平、Casepase-3蛋白表达、BNIP3蛋白表达、BNIP3 mRNA表达水平均升高,且具有剂量-效应关系。结论 砷可致BNIP3高表达,且与L-02细胞凋亡情况存在剂量-效应关系,BNIP3介导了NaAsO2对L-02细胞凋亡的影响。
Abstract:Objective To investigate the effect of sodium arsenite(NaAsO2) on apoptosis in normal human liver L-02 cells and to elucidate the role of BNIP3 in NaAsO_2-induced apoptosis.Methods L-02 cells were treated with different concentrations of NaAsO2(0 μM,5 μM,10 μM,20 μM,40 μM) for 24 hours.Total cellular protein was extracted.Apoptosis levels were assessed using flow cytometry.The expression of Caspase-3 and BNIP3 proteins was detected by Western blotting, and BNIP3 mRNA expression was measured by quantitative PCR(qPCR).Results With increasing concentrations of sodium arsenite, levels of apoptosis, Caspase-3 protein, BNIP3 protein, and BNIP3 mRNA in L-02 cells all increased in a dose-dependent manner.Conclusion Sodium arsenite induces high expression of BNIP3,which is positively correlated with the degree of apoptosis in L-02 cells.These findings suggest that BNIP3 mediates sodium arsenite-induced apoptosis in hepatocytes.
[1] 沈雁峰,孙殿军,赵新华,等.中国饮水型地方性砷中毒病区和高砷区水砷筛查报告[J].中国地方病学杂志,2005,24(2):172-175.
[2] 荀黎红,吴丛雅.地方性砷中毒与人体多系统损害的研究进展[J].国外医学(医学地理分册),1998,19(4):145-156.
[3] 马艳,玉建,吴顺华.砷对神经系统损伤的研究进展[J].中国地方病学杂志.2007,26(1):111-113.
[4] NAGARJUNA VASAGIRI,VIJAY KUMAR KUTALA.Structure,function,and epigenetic regulation of BNIP3:a pathophysiological relevance[J].Mol Biol Rep,2014,41:7705-7714.
[5] LI C,GUAN T,CHEN X,et al.BNIP3 mediates pre-myelinating oligodendrocyte cell death in hypoxia and ischemia[J].J Neurochem,2013,127(3):426-433.
[6] YARON AVIV,JAMES SHAW,HONGYING GANG,et al.Regulation of Autophagy in the Heart:“You Only Live Twice”[J].Antioxidants & Redox Signaling,2011,14(11):2245-2249.
[7] 谢晋予,郑长黎.BNIP3及其与肿瘤的关系[J].国际病理科学与临床杂志,2006,26(4):298-301.
[8] 曾浩,黄颖,陈铌,等.促凋亡线粒体蛋白BNIP3的表达调控与功能[J].现代泌尿生殖肿瘤杂志,2010,2(2):117-120.
[9] 胡亚男,赵巍,陈承志,等.NaAsO2和三氧化二砷对人正常肝细胞增殖和凋亡效应的影响[J].卫生研究,2014,43(2):203-209.
[10] 高艳芳,郭有,欧阳璐,等.NaAsO2致人肝细胞凋亡及NF-kB信号通路影响的研究[J].毒理学杂志,2016,30(5):346-349.
基本信息:
DOI:
中图分类号:R994
引用信息:
[1]潘庆礼.BNIP3在含砷化合物致肝细胞凋亡中的介导作用[J].微量元素与健康研究,2025,42(04):1-3+17.
基金信息: